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CONTACT INFORMATION
Dr. Jason C. Hurlbert
301B Sims Science Bldg
Rock Hill, SC 29733, USA
803/323-4928
803/323-2246 (fax)

CHEM525: Biochemistry Laboratory Techniques (Fall 2013)

Experiment Lectures

Week of Topic / Procedure
Aug 19 Course Overview and Semester Plan
Aug 26

1) Practical Bioinformatics: Looking AND Seeing
Sept 2

2) Spectrophotometric Assays
Sept 9

3) Protein Expression and Purification:

Experiment 3a: Cell Lysis
Sept 16

3) Protein Expression and Purification:

Experiment 3b: FPLC: Ion Exchange Chromatography
Sept 23

4) Analysis of Protein Purification: SDS-PAGE and Western Blot
Sept 30

5) Analysis of Depolymerization Products by HPLC
Oct 7

6) Kinetics by Spectrophotometry I: Influence of [Substrate] on enzymatic reactions
Oct 14 No Class
Oct 21

7) Kinetics by Spectrophotometry II: Influence of pH on enzymatic reactions
Oct 28 8)Kinetics by Spectrophotometry III: Chemical Denaturation of Proteins
Nov 4

 
Nov 11 9) Mass Spectrometry I: MALDI-TOF MS analysis of purified FPLC fractions
Nov 18

Final Report Preparation
Nov 25

Thanksgiving Break

Prelab Session Will Meet
Lab Sections Will Not Meet
Dec 2 Final Exam
Dec 9 Final Lab Report Due by 3 PM

(FLABB = Fundamental Laboratory Approaches for Biochemistry and Biotechnology)