Protocols and Operating Procedures
Links to the protocols and standard operating procedures used in the lab are below. As usual, trust your eyes and hands before blindly following a protocol. Check the reagents we have in stock and be certain that everything matches.
Intrumentation
Voyager DE-STR MALDI-TOF mass spectrometer
Beckman DU-800 Spectrophotometer
Any departmental Centrifuge
Biological Safety Cabinet
Fisher Scientific Accumet AB15 Calibration
Protocols
Alkaline Lysis Miniprep
Calcium Competent Cell Production
Bacterial transformation (Calcium competent cells)
Cell culture
SDS-PAGE
Western Blotting (Semi-dry)
Sample Cleanup with Zip Tips prior to MALDI-TOF MS analysis
Optimizing MALDI-TOF Mass Spectra
Post-source decay experiments on the MALDI-TOF
In-gel trypsin digestion and analysis by MALDI-TOF
|
