Dr. Jason C. Hurlbert, 301B Sims Science Building , Rock Hill, SC  29733    803/323-4928    803/323-2246 (Fax)   
Hurlbert Lab Home
Useful Programs

Protocols and Operating Procedures

Links to the protocols and standard operating procedures used in the lab are below. As usual, trust your eyes and hands before blindly following a protocol. Check the reagents we have in stock and be certain that everything matches.


Voyager DE-STR MALDI-TOF mass spectrometer

Beckman DU-800 Spectrophotometer

Any departmental Centrifuge

Biological Safety Cabinet

Fisher Scientific Accumet AB15 Calibration



Alkaline Lysis Miniprep

Calcium Competent Cell Production

Bacterial transformation (Calcium competent cells)

Cell culture


Western Blotting (Semi-dry)

Sample Cleanup with Zip Tips prior to MALDI-TOF MS analysis

Optimizing MALDI-TOF Mass Spectra

Post-source decay experiments on the MALDI-TOF

In-gel trypsin digestion and analysis by MALDI-TOF












Rock Hill, South Carolina   29733
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