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Dr. Jason C. Hurlbert, 301B Sims Science Building , Rock Hill, SC  29733    803/323-4928    803/323-2246 (Fax)   
Hurlbert Lab Home
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Protocols and Operating Procedures

Links to the protocols and standard operating procedures used in the lab are below. As usual, trust your eyes and hands before blindly following a protocol. Check the reagents we have in stock and be certain that everything matches.

Intrumentation

Voyager DE-STR MALDI-TOF mass spectrometer

Beckman DU-800 Spectrophotometer

Any departmental Centrifuge

Biological Safety Cabinet

Fisher Scientific Accumet AB15 Calibration

 

Protocols

Alkaline Lysis Miniprep

Calcium Competent Cell Production

Bacterial transformation (Calcium competent cells)

Cell culture

SDS-PAGE

Western Blotting (Semi-dry)

Sample Cleanup with Zip Tips prior to MALDI-TOF MS analysis

Optimizing MALDI-TOF Mass Spectra

Post-source decay experiments on the MALDI-TOF

In-gel trypsin digestion and analysis by MALDI-TOF

 

 

 

 

 

 

 

 

 

 

 

Rock Hill, South Carolina   29733
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