Lsn 6: Enzymes as Drug Targets I  (Reaction kinetics, equilibrium constants, binding interactions)

• Chemical kinetics
• Understand the two fundamental requirements for a chemical reaction to occur…
• Draw reaction coordinate-energy profiles and clearly label activation energies, and heat gained or lost
• Understand and be able to clearly explain the role of catalysts
• Understand and be able to use fundamental principles to clearly describe the dependence of reaction rate changes with temperature
• Arrehenius equation
• Know and be able to use the Arrehenius equation for rate constant determination
• Demonstrated the ability to calculate relative rates of reactions for different activation energies or temperatures
• Understand how to experimentally determine a reaction's activation energy using Arrhenius plots
• Understand relative impacts of temperature changes on rates for chemical reactions with low and high activation energies
• Be able to use activiation energies to predict rate constants at different temperatures
• Rate law
• Understand what is meant by the rate law and outline the experimental procedures and methodology to determine reaction order
• Use a given rate law to calculate rate constants and their associated units
• Enzyme-substrate interactions
• Be able to draw and to clearly explain reaction energy diagrams for enzyme-substrate interactions
• Understand the effects of inhibitors and what is specifically meant by IC50
• Understand and clearly explain the basis for important types of noncovalent enzyme-substrate interactions
• Clearly describe the enzyme inhibition process