Lesson 8: Enzymes

• Chemical kinetics
• Understand what is meant by the rate law and outline the experimental procedures and methodology to determine reaction order
• Use a given rate law to calculate rate constants and their associated units
• Understand how to experimentally determine a reaction's activation energy using Arrhenius plots
• Be able to use activiation energies to predict rate constants at different temperatures
• Understand the effect of activation energy on the degree of rate constant changes with temperature
• Michaelis-Menton kinetics
• Write equilibria associated with enzyme-substrate interactions
• Understand how reaction order changes with substrate concentration
• Be able to use the Lineweaver-Burke relationship to calculate V_max, turnover number, and K_M
• Understand the significance of K_M; understand the relation of K_M to enzyme-substrate complex stability and to maximum reaction rate
• Enzyme-substrate interactions
• Be able to draw and to clearly explain reaction energy diagrams for enzyme-substrate interactions
• Understand the effects of inhibitors and what is specifically meant by IC50
• Understand and clearly explain the basis for important types of noncovalent enzyme-substrate interactions
• Clearly describe the enzyme inhibition process