Thursday, January 13: Chapter 1 (Intermolcular Forces and the Hydrophobic Effect)
Most important concepts from today's lecture: Importance of the form-function relationship in biochemistry, what the 4 IMFs are, how they differ in energy as a function of distance, ordered water and the hydrophobic effect, the Henderson-Hasselbach equation and what the pKa of a compound means.
Tuesday, January 18: Chapter 2 Part One (Amino acids and secondary structures)
Important concepts from today's lecture: The common backbone of all amino acids, the structure, name and both 1 and 3-letter abbreviations of the amino acids, the protonation state of amino acids at physiological pH (the alpha-amino group, the alpha-carboxylic acid group and the side chains of ionizable amino acids), the 7 most important amino acids to learn RIGHT NOW and the formation of a peptide bond by a condensation reaction.
Thursday, January 20: Chapter 2 Part 2 (The peptide bond, Ramachandran plots, primary, secondary and tertiary structures)
Important concepts from this lecture: Do not wait until the night before the test to start drawing the amino acids, knowing their chenical properties (hydrophobic, polar uncharged, polar charged, acidic, basic) and their one and three letter abbreviations, What makes a peptide bond special, what is a Ramachandran plot (Yeah, you should be able to draw it), the difference between levels of protein structure, what are the types of protein secondaray structure.
Tuesday, January 25: Chapter 2 Part 3 (The Anfinsen Experiment and Protein Folding) and Chapter 3 Part 1: Chromatographic methods for protein purification.
Important concepts from this lecture: The four levels of protein structure and the IMFs associated with each, the Anfinsen experiment and how it showed that the primary structure of a protein determines its tertiary structure, the steps involved in the process of protein folding, Three types of column chromatography for the purification of proteins.
Thursday, January 27: Chapter 3 Part 2
Important concepts from this lecture: SDS-PAGE and what it tells you about a protein sample, Isoelectric focusing, Two-dimensional gel electrophoresis, antibody structure, ELISAs and Western blotting.
Tuesday, February 1: Chapter 3 Part 3
Important Concepts from this lecture: Review MALDI-TOF mass spectrometry in your textbook, the Edman degradation and what its products are, using site-specific proteases and the Edman degradation to sequence peptides.
Thursday, February 3: Chapter 7 Part 1
Important concepts from today's lecture: The function of myoglobin and hemoglobin, why hemoglobin displays cooperative binding with respect to its function (and, conversely, why myoglobin doesn't).
Tuesday, February 8: Chapter 8 Part 1
Important concepts from today's lecture: What an enzyme is, teasing apart the binding and catalytic components of an active site, the induced fit model of enzyme action and how that can help lower the activation energy, the concept of the free energy of binding and how it lowers the activation energy.
Thursday, February 10: Chapter 8 Part 2
Important concepts from today's lecture: Reaction rates, how to measure them in the lab, how to plot the data, the similarity between a Product as a function of time plot and an Observed Velocity as a function of substrate concentration (although the latter comes from multiple iterations of the former), the steady state assumption, the Michaelis-Menton euqation and plot, the Lineweaver-Burk form of the Michaelis-Menton equation and its plot, the meaning of the kinetic constants: Vm, Km, kcat, (kcat/Km) and competitive inhibition.
Tuesday, 15 February: Chapter 8 Part 3
Important concepts from today's lecture: The three types of noncovalent inhibition of enzymes, the Michaelis-Menton and Lineweaver-Burk equations and plot of each type of inhibition, what forms of the enzyme the inhibitors bind.
Thursday, February 17: Chapter 8 Finale
Tuesday, February 22: Chapter 9 Part 1
Thursday, February 24: Chapter 9 Part 2
Tuesday, March 1: Chapter 10
Thursday, March 3: Chapter 11
Tuesday, March 8: Chapter 11 Part 2 and Chapter 12 Part 1
Thursday, March 10: Chapter 12 Part 2
Important concepts from today's lecture: Integral and peripheral membrane associated proteins, Membrane fluidity and how organisms can change it, the fluid mosaic model and the evolutionary conundrum it poses for cells responding to environmental stimuli, lipid rafts.
Tuesday, March 22: Chapter 14 Part 1: Signal Transduction
Important Concepts from today's lecture: 5 features every siganl transduction pathway has, two of the five different types of signal transduction systems, the berta-adrenergic system.
Lecture Canceled: Thursday, March 24: Chapter 14 Part 2: Signal Transduction Tyrosine Kinases
Tuesday, March 29: Chapter 14 Part 2: Signal Transduction
Thursday, March 31: Chapter 15 Part 1: Energy Metabolism
Tuesday, April 5: Chapter 15 Part 2: Energy Metabolism
Chapter 16 Part 1: Stage 1 of Glycolysis
Thursday, April 7: Chapter 16 Part 2: Stage 2 of Glycolysis and Regulation of Glycolysis
Tuesday, April 12: Chapter 17: Citric Acid Cycle Part 1
Thursday, April 14: Chapter 17: Citric Acid Cycle Part 2
Tuesday, April 19: Chapter 18: Electron Transport and Oxidative Phosphorylation Part 1
Thursday, April 21: F1F0 ATPase