Processing 1 helix: Transmembrane helix 1 from 68 (in) to 98 (out) : 2937.95 Score = 2.937949 Processing 1 helix: Transmembrane helix 1 from 68 (out) to 98 (in) : 2928.45 Score = 2.928451 Processing 14 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : -3926.38 Transmembrane helix 2 from 34 (out) to 49 (in) : -623.538 Transmembrane helix 3 from 53 (in) to 68 (out) : 360.979 Transmembrane helix 4 from 72 (out) to 87 (in) : 1991.27 Transmembrane helix 5 from 91 (in) to 106 (out) : 546.042 Transmembrane helix 6 from 110 (out) to 125 (in) : 916.183 Transmembrane helix 7 from 129 (in) to 144 (out) : -584.598 Transmembrane helix 8 from 148 (out) to 163 (in) : -744.832 Transmembrane helix 9 from 167 (in) to 182 (out) : -767.658 Transmembrane helix 10 from 186 (out) to 201 (in) : -716.958 Transmembrane helix 11 from 205 (in) to 220 (out) : -903.562 Transmembrane helix 12 from 224 (out) to 239 (in) : -930.835 Transmembrane helix 13 from 243 (in) to 258 (out) : -872.156 Transmembrane helix 14 from 262 (out) to 277 (in) : -681.065 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 27 (out) to 42 (in) : -841.24 Transmembrane helix 2 from 46 (in) to 61 (out) : 544.57 Transmembrane helix 3 from 65 (out) to 80 (in) : 1153.53 Transmembrane helix 4 from 84 (in) to 99 (out) : 1573.21 Transmembrane helix 5 from 103 (out) to 118 (in) : 305.284 Transmembrane helix 6 from 122 (in) to 137 (out) : 549.376 Transmembrane helix 7 from 148 (out) to 163 (in) : -744.832 Transmembrane helix 8 from 167 (in) to 182 (out) : -767.658 Transmembrane helix 9 from 186 (out) to 201 (in) : -716.958 Transmembrane helix 10 from 205 (in) to 220 (out) : -903.562 Transmembrane helix 11 from 224 (out) to 239 (in) : -930.835 Transmembrane helix 12 from 243 (in) to 258 (out) : -872.156 Transmembrane helix 13 from 262 (out) to 277 (in) : -681.065 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 27 (in) to 42 (out) : -838.06 Transmembrane helix 2 from 46 (out) to 61 (in) : 555.23 Transmembrane helix 3 from 65 (in) to 80 (out) : 1149.07 Transmembrane helix 4 from 84 (out) to 99 (in) : 1569.99 Transmembrane helix 5 from 103 (in) to 118 (out) : 296.916 Transmembrane helix 6 from 122 (out) to 137 (in) : 553.324 Transmembrane helix 7 from 150 (in) to 165 (out) : -633.658 Transmembrane helix 8 from 169 (out) to 184 (in) : -803.192 Transmembrane helix 9 from 188 (in) to 203 (out) : -733.039 Transmembrane helix 10 from 214 (out) to 229 (in) : -766.772 Transmembrane helix 11 from 242 (in) to 257 (out) : -878.716 Transmembrane helix 12 from 261 (out) to 276 (in) : -673.739 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 44 (out) to 59 (in) : 619.614 Transmembrane helix 2 from 64 (in) to 79 (out) : 1021.76 Transmembrane helix 3 from 83 (out) to 98 (in) : 1694.79 Transmembrane helix 4 from 102 (in) to 117 (out) : 245.779 Transmembrane helix 5 from 121 (out) to 136 (in) : 692.331 Transmembrane helix 6 from 150 (in) to 165 (out) : -633.658 Transmembrane helix 7 from 169 (out) to 184 (in) : -803.192 Transmembrane helix 8 from 188 (in) to 203 (out) : -733.039 Transmembrane helix 9 from 214 (out) to 229 (in) : -766.772 Transmembrane helix 10 from 242 (in) to 257 (out) : -878.716 Transmembrane helix 11 from 261 (out) to 276 (in) : -673.739 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 44 (in) to 59 (out) : 605.986 Transmembrane helix 2 from 64 (out) to 79 (in) : 1027.84 Transmembrane helix 3 from 83 (in) to 98 (out) : 1699.01 Transmembrane helix 4 from 102 (out) to 117 (in) : 251.521 Transmembrane helix 5 from 121 (in) to 136 (out) : 688.469 Transmembrane helix 6 from 158 (out) to 173 (in) : -593.513 Transmembrane helix 7 from 187 (in) to 202 (out) : -712.877 Transmembrane helix 8 from 214 (out) to 229 (in) : -766.772 Transmembrane helix 9 from 242 (in) to 257 (out) : -878.716 Transmembrane helix 10 from 261 (out) to 276 (in) : -673.739 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 44 (out) to 59 (in) : 619.614 Transmembrane helix 2 from 64 (in) to 79 (out) : 1021.76 Transmembrane helix 3 from 83 (out) to 98 (in) : 1694.79 Transmembrane helix 4 from 102 (in) to 117 (out) : 245.779 Transmembrane helix 5 from 121 (out) to 136 (in) : 692.331 Transmembrane helix 6 from 153 (in) to 168 (out) : -593.846 Transmembrane helix 7 from 187 (out) to 202 (in) : -710.823 Transmembrane helix 8 from 214 (in) to 229 (out) : -774.428 Transmembrane helix 9 from 260 (out) to 275 (in) : -664.665 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 44 (in) to 59 (out) : 605.986 Transmembrane helix 2 from 64 (out) to 79 (in) : 1027.84 Transmembrane helix 3 from 83 (in) to 98 (out) : 1699.01 Transmembrane helix 4 from 102 (out) to 117 (in) : 251.521 Transmembrane helix 5 from 121 (in) to 136 (out) : 688.469 Transmembrane helix 6 from 158 (out) to 173 (in) : -593.513 Transmembrane helix 7 from 187 (in) to 202 (out) : -712.877 Transmembrane helix 8 from 260 (out) to 275 (in) : -664.665 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 44 (out) to 59 (in) : 619.614 Transmembrane helix 2 from 64 (in) to 79 (out) : 1021.76 Transmembrane helix 3 from 83 (out) to 98 (in) : 1694.79 Transmembrane helix 4 from 102 (in) to 117 (out) : 245.779 Transmembrane helix 5 from 121 (out) to 136 (in) : 692.331 Transmembrane helix 6 from 153 (in) to 168 (out) : -593.846 Transmembrane helix 7 from 260 (out) to 275 (in) : -664.665 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 44 (in) to 59 (out) : 605.986 Transmembrane helix 2 from 64 (out) to 79 (in) : 1027.84 Transmembrane helix 3 from 83 (in) to 98 (out) : 1699.01 Transmembrane helix 4 from 102 (out) to 117 (in) : 251.521 Transmembrane helix 5 from 121 (in) to 136 (out) : 688.469 Transmembrane helix 6 from 158 (out) to 173 (in) : -593.513 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 44 (out) to 59 (in) : 619.614 Transmembrane helix 2 from 64 (in) to 79 (out) : 1021.76 Transmembrane helix 3 from 83 (out) to 98 (in) : 1694.79 Transmembrane helix 4 from 102 (in) to 117 (out) : 245.779 Transmembrane helix 5 from 121 (out) to 136 (in) : 692.331 Score = 4.314496 Processing 4 helices: Transmembrane helix 1 from 44 (in) to 59 (out) : 605.986 Transmembrane helix 2 from 65 (out) to 88 (in) : 2292.51 Transmembrane helix 3 from 92 (in) to 107 (out) : 471.471 Transmembrane helix 4 from 112 (out) to 130 (in) : 1244.26 Score = 4.576986 Processing 3 helices: Transmembrane helix 1 from 44 (out) to 59 (in) : 619.614 Transmembrane helix 2 from 68 (in) to 98 (out) : 2937.95 Transmembrane helix 3 from 112 (out) to 130 (in) : 1244.26 Score = 4.857458 Processing 2 helices: Transmembrane helix 1 from 68 (in) to 98 (out) : 2937.95 Transmembrane helix 2 from 112 (out) to 130 (in) : 1244.26 Score = 4.197442 Processing 14 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : -3927.12 Transmembrane helix 2 from 34 (in) to 49 (out) : -626.962 Transmembrane helix 3 from 53 (out) to 68 (in) : 359.121 Transmembrane helix 4 from 72 (in) to 87 (out) : 1993.73 Transmembrane helix 5 from 91 (out) to 106 (in) : 538.158 Transmembrane helix 6 from 110 (in) to 125 (out) : 911.217 Transmembrane helix 7 from 129 (out) to 144 (in) : -586.302 Transmembrane helix 8 from 148 (in) to 163 (out) : -737.368 Transmembrane helix 9 from 167 (out) to 182 (in) : -767.142 Transmembrane helix 10 from 186 (in) to 201 (out) : -717.642 Transmembrane helix 11 from 205 (out) to 220 (in) : -902.338 Transmembrane helix 12 from 224 (in) to 239 (out) : -937.865 Transmembrane helix 13 from 243 (out) to 258 (in) : -871.244 Transmembrane helix 14 from 262 (in) to 277 (out) : -677.035 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 27 (in) to 42 (out) : -838.06 Transmembrane helix 2 from 46 (out) to 61 (in) : 555.23 Transmembrane helix 3 from 65 (in) to 80 (out) : 1149.07 Transmembrane helix 4 from 84 (out) to 99 (in) : 1569.99 Transmembrane helix 5 from 103 (in) to 118 (out) : 296.916 Transmembrane helix 6 from 122 (out) to 137 (in) : 553.324 Transmembrane helix 7 from 148 (in) to 163 (out) : -737.368 Transmembrane helix 8 from 167 (out) to 182 (in) : -767.142 Transmembrane helix 9 from 186 (in) to 201 (out) : -717.642 Transmembrane helix 10 from 205 (out) to 220 (in) : -902.338 Transmembrane helix 11 from 224 (in) to 239 (out) : -937.865 Transmembrane helix 12 from 243 (out) to 258 (in) : -871.244 Transmembrane helix 13 from 262 (in) to 277 (out) : -677.035 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 27 (out) to 42 (in) : -841.24 Transmembrane helix 2 from 46 (in) to 61 (out) : 544.57 Transmembrane helix 3 from 65 (out) to 80 (in) : 1153.53 Transmembrane helix 4 from 84 (in) to 99 (out) : 1573.21 Transmembrane helix 5 from 103 (out) to 118 (in) : 305.284 Transmembrane helix 6 from 122 (in) to 137 (out) : 549.376 Transmembrane helix 7 from 151 (out) to 166 (in) : -613.134 Transmembrane helix 8 from 170 (in) to 185 (out) : -822.268 Transmembrane helix 9 from 189 (out) to 204 (in) : -739.393 Transmembrane helix 10 from 214 (in) to 229 (out) : -774.428 Transmembrane helix 11 from 243 (out) to 258 (in) : -871.244 Transmembrane helix 12 from 262 (in) to 277 (out) : -677.035 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 44 (in) to 59 (out) : 605.986 Transmembrane helix 2 from 64 (out) to 79 (in) : 1027.84 Transmembrane helix 3 from 83 (in) to 98 (out) : 1699.01 Transmembrane helix 4 from 102 (out) to 117 (in) : 251.521 Transmembrane helix 5 from 121 (in) to 136 (out) : 688.469 Transmembrane helix 6 from 151 (out) to 166 (in) : -613.134 Transmembrane helix 7 from 170 (in) to 185 (out) : -822.268 Transmembrane helix 8 from 189 (out) to 204 (in) : -739.393 Transmembrane helix 9 from 214 (in) to 229 (out) : -774.428 Transmembrane helix 10 from 243 (out) to 258 (in) : -871.244 Transmembrane helix 11 from 262 (in) to 277 (out) : -677.035 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 44 (out) to 59 (in) : 619.614 Transmembrane helix 2 from 64 (in) to 79 (out) : 1021.76 Transmembrane helix 3 from 83 (out) to 98 (in) : 1694.79 Transmembrane helix 4 from 102 (in) to 117 (out) : 245.779 Transmembrane helix 5 from 121 (out) to 136 (in) : 692.331 Transmembrane helix 6 from 150 (in) to 165 (out) : -633.658 Transmembrane helix 7 from 169 (out) to 184 (in) : -803.192 Transmembrane helix 8 from 188 (in) to 203 (out) : -733.039 Transmembrane helix 9 from 214 (out) to 229 (in) : -766.772 Transmembrane helix 10 from 260 (in) to 275 (out) : -665.535 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 44 (in) to 59 (out) : 605.986 Transmembrane helix 2 from 64 (out) to 79 (in) : 1027.84 Transmembrane helix 3 from 83 (in) to 98 (out) : 1699.01 Transmembrane helix 4 from 102 (out) to 117 (in) : 251.521 Transmembrane helix 5 from 121 (in) to 136 (out) : 688.469 Transmembrane helix 6 from 158 (out) to 173 (in) : -593.513 Transmembrane helix 7 from 187 (in) to 202 (out) : -712.877 Transmembrane helix 8 from 214 (out) to 229 (in) : -766.772 Transmembrane helix 9 from 260 (in) to 275 (out) : -665.535 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 44 (out) to 59 (in) : 619.614 Transmembrane helix 2 from 64 (in) to 79 (out) : 1021.76 Transmembrane helix 3 from 83 (out) to 98 (in) : 1694.79 Transmembrane helix 4 from 102 (in) to 117 (out) : 245.779 Transmembrane helix 5 from 121 (out) to 136 (in) : 692.331 Transmembrane helix 6 from 153 (in) to 168 (out) : -593.846 Transmembrane helix 7 from 187 (out) to 202 (in) : -710.823 Transmembrane helix 8 from 260 (in) to 275 (out) : -665.535 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 44 (in) to 59 (out) : 605.986 Transmembrane helix 2 from 64 (out) to 79 (in) : 1027.84 Transmembrane helix 3 from 83 (in) to 98 (out) : 1699.01 Transmembrane helix 4 from 102 (out) to 117 (in) : 251.521 Transmembrane helix 5 from 121 (in) to 136 (out) : 688.469 Transmembrane helix 6 from 158 (out) to 173 (in) : -593.513 Transmembrane helix 7 from 260 (in) to 275 (out) : -665.535 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 44 (out) to 59 (in) : 619.614 Transmembrane helix 2 from 64 (in) to 79 (out) : 1021.76 Transmembrane helix 3 from 83 (out) to 98 (in) : 1694.79 Transmembrane helix 4 from 102 (in) to 117 (out) : 245.779 Transmembrane helix 5 from 121 (out) to 136 (in) : 692.331 Transmembrane helix 6 from 153 (in) to 168 (out) : -593.846 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 44 (in) to 59 (out) : 605.986 Transmembrane helix 2 from 64 (out) to 79 (in) : 1027.84 Transmembrane helix 3 from 83 (in) to 98 (out) : 1699.01 Transmembrane helix 4 from 102 (out) to 117 (in) : 251.521 Transmembrane helix 5 from 121 (in) to 136 (out) : 688.469 Score = 4.232605 Processing 4 helices: Transmembrane helix 1 from 44 (out) to 59 (in) : 619.614 Transmembrane helix 2 from 65 (in) to 88 (out) : 2292.29 Transmembrane helix 3 from 92 (out) to 107 (in) : 463.329 Transmembrane helix 4 from 112 (in) to 130 (out) : 1238.74 Score = 4.651215 Processing 3 helices: Transmembrane helix 1 from 44 (in) to 59 (out) : 605.986 Transmembrane helix 2 from 68 (out) to 98 (in) : 2928.45 Transmembrane helix 3 from 112 (in) to 130 (out) : 1238.74 Score = 4.717542 Processing 2 helices: Transmembrane helix 1 from 68 (out) to 98 (in) : 2928.45 Transmembrane helix 2 from 112 (in) to 130 (out) : 1238.74 Score = 4.151958 Summary of topology analysis: 1 helix (+) : Score = 2.93795 1 helix (-) : Score = 2.92845 2 helices (+) : Score = 4.19744 2 helices (-) : Score = 4.15196 3 helices (+) : Score = 4.71754 3 helices (-) : Score = 4.85746 4 helices (+) : Score = 4.57699 4 helices (-) : Score = 4.65122 5 helices (+) : Score = 4.2326 5 helices (-) : Score = 4.3145 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 Results: Signal peptide: 1-40 Signal score: 18.672 Topology: 44-59,68-98,112-130 Re-entrant helices: Not detected. Helix count: 3 N-terminal: out Score: 4.85746